德州撲克技巧攻略eLife:擬南芥TIR
Genetic analysis of the Arabidopsis TIR1/AFB auxin receptors reveals both overlapping and specialized functionsFirst author: Michael J Prigge; Affiliations: University of California錛 San Diego (加州大學聖地亞哥分校): La Jolla錛 USACorresponding author: Mark EstelleThe TIR1/AFB auxin co-receptors mediate diverse responses to the plant hormone auxin. The Arabidopsis genome encodes six TIR1/AFB proteins representing three of the four clades that were established prior to angiosperm radiation. To determine the role of these proteins in plant development we performed an extensive genetic analysis involving the generation and characterization of all possible multiply-mutant lines. We find that loss of all six TIR1/AFB proteins results in early embryo defects and eventually seed abortion錛 and yet a single wild-type allele of TIR1 or AFB2 is sufficient to support growth throughout development. Our analysis reveals extensive functional overlap between even the most distantly related TIR1/AFB genes except for AFB1. Surprisingly錛 AFB1 has a specialized function in rapid auxin-dependent inhibition of root growth and early phase of root gravitropism. This activity may be related to a difference in subcellular localization compared to the other members of the family.TIR1/AFB生長素共受體介導植物對于生長素的多種響應。擬南芥基因組編碼六個TIR1/AFB蛋白,分屬在被子植物輻射之前就已經形成的四大類中的三類。為了研究這些TIR1/AFB蛋白在植物發育過程中的作用,作者構建了所有可能的多突變株系,通過大規模的遺傳學分析對這些蛋白進行功能鑑定。作者發現6個TIR1/AFB蛋白同時功能丟失會導致胚胎早期的發育缺陷,最終引起種子敗育,然而單個TIR1或者AFB2野生型等位基因回補六突植株足以保證其完成生活史。作者的分析顯示除了AFB1基因之前,其它所有的TIR1/AFB蛋白即使是親緣關系最遠的兩個蛋白之間,